Prenatal diagnosis trials--fetal cells/maternal blood

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Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Project Description:
This project represents phase II of a project whose main Objective is to develOp a non-invasive, safe, relatively inexpensive and accurate technique for the prenatal diagnosis Of genetic disorders that can be performed during the first trimester. Phase II will test, expand and refine the methodology developed in phase I. The study will include a systematic evaluation of the variables involved in separating and enriching fetal cells from maternal blood through flow cytometry or other methods, including magnetic-activated cell sorting (MACS), followed by in situ hybridization with chromosome-specific DNA probes. The results of these peripheral blood studies will be compared to those that will be obtained from amniocentesis or chorionic villus sampling (CVS) on the same women. Another objective of the project is to determine whether or not there are any non-biological effects on the women undergoing prenatal diagnostic testing. Even if the biologic risks associated with reproductive genetic technologies are reduced, the possibility exists that other potential risks (or benefits) are associated with the procedures. Some of these factors may be: expanded or diminished maternal anxiety, increased adjustment or maladaptation to the pregnancy, increased feelings of coercion to undertake prenatal testing when there are negligible biologic risks associated with the procedure, and increased or decreased comfort with reproductive decision-making.
 
Project Terms:
pregnancy circulation prenatal diagnosis diagnosis design /evaluation genetic disorder diagnosis nucleic acid probes chromosome aberrations fluorescent dye /probe diagnosis quality /standard clinical trials noninvasive diagnosis female psychological tests flow cytometry in situ hybridization human fetus tissue human pregnant subject
Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Project Categories:
• Natural Sciences > Experimental techniques, Aging Diseases and Pathology > in vitro methods, Cardiovascular and cerebrovascular diseases > Flow cytometry, Heart failure
 
Other Information:
Fiscal Year: 1994
Project Start Date: 15 March 1994
Project End Date: 30 November 2002
Administering Institute Or Center: HD
 
Project Funding Information:
Total Funding: $970,355
Year Funding Organization Total Funding, $
2000 EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT $970,355
Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Project_number Title Year FY Total Cost
There are no results for this project in database.
Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Project number Project title Organization FY Funding Organization FY Total Cost
N01HD043202-009PRENATAL DIAGNOSIS TRIALS--FETAL CELLS/MATERNAL BLOODWAYNE STATE UNIVERSITY2000EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
$970,355
Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Project number Project title Principal investigator
There are no any related projects.
Project Title: Prenatal diagnosis trials--fetal cells/maternal blood
Principal Investigators (PI): EVANS MARK I
Project Number: N01HD043202-009
Organization: WAYNE STATE UNIVERSITY
 
Title Abstract Authors Year Rel
An examination of different Percoll density gradients and magnetic activated cell sorting (MACS) for the enrichment of fetal erythroblasts from maternal blood. Archives of gynecology and obstetrics. 2000 Apr 263 (4) :160-3 Smits, G; Holzgreve, W; Hahn, S 2000
Multiplex and real-time quantitative PCR on fetal DNA in maternal plasma. A comparison with fetal cells isolated from maternal blood. Annals of the New York Academy of Sciences. 2000 Apr 906 () :148-52 Hahn, S; Zhong, X Y; Burk, M R; Troeger, C; Holzgreve, W 2000
Fetal cells in cervical mucus and maternal blood. Bailliere's best practice & research. Clinical obstetrics & gynaecology. 2000 Aug 14 (4) :709-22 Holzgreve, W; Hahn, S 2000
Fetal DNA in maternal plasma is elevated in pregnancies with aneuploid fetuses. Prenatal diagnosis. 2000 Oct 20 (10) :795-8 Zhong, X Y; Burk, M R; Troeger, C; Jackson, L R; Holzgreve, W; Hahn, S 2000
High levels of fetal erythroblasts and fetal extracellular DNA in the peripheral blood of a pregnant woman with idiopathic polyhydramnios: case report. Prenatal diagnosis. 2000 Oct 20 (10) :838-41 Zhong, X Y; Holzgreve, W; Li, J C; Aydinli, K; Hahn, S 2000
Detection of fetal Rhesus D and sex using fetal DNA from maternal plasma by multiplex polymerase chain reaction. BJOG : an international journal of obstetrics and gynaecology. 2000 Jun 107 (6) :766-9 Zhong, X Y; Holzgreve, W; Hahn, S 2000
Fluctuation of maternal and fetal free extracellular circulatory DNA in maternal plasma. Obstetrics and gynecology. 2000 Dec 96 (6) :991-6 Zhong, X Y; Burk, M R; Troeger, C; Kang, A; Holzgreve, W; Hahn, S 2000