Core--animal model and cell

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Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Project Description:
1. Purpose, Core C, the animal model and cell isolation core, provides support for the central animal model used by Projects 1-4. This includes expert surgical instrumentation, follow-up care, and isolation of viable cardiomyocytes from several layers of the left ventricle. Myocytes are employed in projects 1-3 for molecular dissection of potassium and calcium channel physiology in normal and failing hearts. Myocytes are utilized in Project 4 for the purpose of studying neurohormonal modulation of ion channels in normal and failing hearts, and test the role of an altered extracellular matrix environment. Chunk tissues are also provided by Core C at time of animal sacrifice and are used for a variety of molecular assays and histochemical analyses. Two models are employed in Core C, the standard 4 week tachycardia-pacing model of dilated cardiomyopathy, and an accelerated model employing combined neurohumoral activation with relatively short-term tachycardia pacing (Project 4). The major roles of the core are 1) to provide a weekly supply of acutely isolated myocytes from control and abnormal (failing) left ventricles; 2) to provide a standardized hemodynamic/organ electrophysiologic characterization of hearts from which these cells are obtained; 3) to provide chronic surgery-instrumentation and post operative care for animals requiring chronic repetitive conscious hemodynamic assessment; and 4) to provide all required medical records for chronic care and animal maintenance, and to administer these protocols following policies set forth by the Animal Care and Use Policies of the Johns Hopkins University pursuant to guidelines established by the National Institute of Health.
 
Project Terms:
cardiac myocytes animal care tissue /cell preparation biomedical facility dogs
Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Project Categories:
• Natural Sciences > Aging Diseases and Pathology > Cancer & related diseases > Malignant neoplasms (including in situ) > Genitourinary cancer > Ovarian cancer
 
Other Information:
Fiscal Year: 1995
Project Start Date: 20 January 1995
Project End Date: 31 December 2004
Administering Institute Or Center: HL
 
Project Funding Information:
Total Funding: $209,733
Year Funding Organization Total Funding, $
2000 NATIONAL HEART, LUNG, AND BLOOD INSTITUTE $209,733
Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Project_number Title Year FY Total Cost
There are no results for this project in database.
Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Project number Project title Organization FY Funding Organization FY Total Cost
5T32HL007227-29Pathophysiology of Myocardial DiseaseJOHNS HOPKINS UNIVERSITY2004NATIONAL HEART
$440,636
5R01HL047511-11Role of Stretch to Endothelial Pulse-Perfusion ResponseJOHNS HOPKINS UNIVERSITY2004NATIONAL HEART
$367,875
1P01HL077180-01-9003CORE A--ADMINISTRATIVE COREJOHNS HOPKINS UNIVERSITY2004NATIONAL HEART
$98,821
1P01HL077180-01-9001CORE--ANIMAL COREJOHNS HOPKINS UNIVERSITY2004NATIONAL HEART
$203,090
1P01HL077180-01Pathobiology of Cardiac Dyssynchrony &ResynchronizationJOHNS HOPKINS UNIVERSITY2004NATIONAL HEART
$2,261,167
5T32HL007227-28Pathophysiology of Myocardial DiseaseJOHNS HOPKINS UNIVERSITY2003NATIONAL HEART
$745,173
5R01HL047511-10Role of Stretch to Endothelial Pulse-Perfusion ResponseJOHNS HOPKINS UNIVERSITY2003NATIONAL HEART
$367,875
2T32HL007227-26Pathophysiology of Myocardial DiseaseJOHNS HOPKINS UNIVERSITY2001NATIONAL HEART
$658,948
5T32HL007227-27Pathophysiology of Myocardial DiseaseJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$663,508
2R01HL047511-09Role of Stretch to Endothelial Pulse-Perfusion ResponseJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$367,875
5P50HL052307-08-9002Core--animal model and cellJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$209,733
3P50HL052307-08S1-9002Core--animal model and cellJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$209,733
5P50HL052307-08-0007Mechanism of neurohumoral modulation of cardiac remodelingJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$209,733
3P50HL052307-08S1-0007Mechanism of neurohumoral modulation of cardiac remodelingJOHNS HOPKINS UNIVERSITY2002NATIONAL HEART
$209,733
5P50HL052307-07-9002Core--animal model and cellJOHNS HOPKINS UNIVERSITY2001NATIONAL HEART
$209,733
5P50HL052307-07-0007Mechanism of neurohumoral modulation of cardiac remodelingJOHNS HOPKINS UNIVERSITY2001NATIONAL HEART
$209,733
5T32HL007227-25PATHOPHYSIOLOGY OF MYOCARDIAL DISEASEJOHNS HOPKINS UNIVERSITY2000NATIONAL HEART
$499,272
5R01HL047511-08PATHOPHYSIOLOGY OF VENTRICULAR-VASCULAR INTERACTIONJOHNS HOPKINS UNIVERSITY2000NATIONAL HEART
$297,855
2P50HL052307-06-9002Core--animal model and cellJOHNS HOPKINS UNIVERSITY2000NATIONAL HEART
$209,733
2P50HL052307-06-0007Mechanism of neurohumoral modulation of cardiac remodelingJOHNS HOPKINS UNIVERSITY2000NATIONAL HEART
$209,733
Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Project number Project title Principal investigator
There are no any related projects.
Project Title: Core--animal model and cell
Principal Investigators (PI): KASS DAVID ALAN
Project Number: 2P50HL052307-06-9002
Parent Project: 2P50HL052307-06
Organization: JOHNS HOPKINS UNIVERSITY
 
Title Abstract Authors Year Rel